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| 1. |  | MARTINS, P. do C.; CARNEIRO, A. V.; ÁLVARES, J. G.; NETO, A.; PIZZOL, T. S.; OLIVEIRA, W. X. de; RIBEIRO, D. B. de C. L.; ARAUJO, P. B. de. Indicadores de desempenho na atividade leiteira: uma abordagem inovadora. In: CONGRESSO INTERNACIONAL DO LEITE, 10.; WORKSHOP SOBRE POLÍTICAS PÚBLICAS, 10.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 11., 2011, Maceió. Anais... Juiz de Fora: Embrapa Gado de Leite, 2011. 3 p. 1 CD-ROM.| Biblioteca(s): Embrapa Gado de Leite. |
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| 2. | | MARTINS, P. do C.; CARNEIRO, A. V.; ÁLVARES, J. G.; OLIVEIRA, W. X. de; NETO, A.; RIBEIRO, D. B. de C. L.; ARAÚJO, P. B. de; SENRA, T. Desempenho financeiro de propriedades leiteiras selecionadas. Boletim CBLeite, Juiz de Fora, v. 5, n. 15, p. 40-42, 2011.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 2 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
08/10/2014 |
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Data da última atualização: |
08/10/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SOUZA, A. L.; ANGELO, P. C. da S.; NOGUEIRA, P. P. O.; GONÇALVES, J. F. C.; FRANCO, A. M.; ASTOLFI-FILHO, S.; LÓPEZ-LOZANO, J. L.; ANDRADE, E. V. |
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Afiliação: |
A. L. Souza, UFAM; PAULA CRISTINA DA SILVA ANGELO, CPAA; P. P. O. Nogueira, FIOCRUZ; J. F. C. Gonçalves, INPA; A. M. Franco, INPA; S. Astolfi-Filho, UFAM; J. L. López-Lozano, Fundação de Medicina Tropical do Amazonas; E. V. Andrade, UFAM. |
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Título: |
Method for obtaining high-resolution proteomic analysis from pericarps of guarana. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 13, n. 3, p. 8014-8024, 2014. |
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DOI: |
http://dx.doi.org/10.4238/2014.September.29.14 |
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Idioma: |
Inglês |
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Conteúdo: |
Guarana has great agricultural potential and is largely used therapeutically and in the production of non-alcoholic energy drinks. Genomic and proteomic studies are crucial to identify proteins that play central roles in the maintenance and viability of fruits, as well as to identify proteins related to the main metabolic pathways. However, the success of any protein analysis starts with the protein extract preparation, which needs to offer an extract that is free of contaminants. This study aimed to evaluate different extraction methods to obtain high-quantity and high-quality extracts that are compatible with analysis by 2-dimensional electrophoresis and tandem mass spectrometry protein identification. Three different methods were tested: trichloroacetic acid (TCA)/acetone, sodium dodecyl sulfate (SDS)/phenol, and polyvinylpolypyrrolidone (PVPP)/SDS/phenol. |
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Palavras-Chave: |
Plant proteomics; Two-dimensional electrophoresis. |
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Thesagro: |
Guaraná; Paullinia Cupana. |
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Thesaurus NAL: |
polyphenols. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/109684/1/gmr4011.pdf
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Marc: |
LEADER 01739naa a2200277 a 4500
001 1996859
005 2014-10-08
008 2014 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/2014.September.29.14$2DOI
100 1 $aSOUZA, A. L.
245 $aMethod for obtaining high-resolution proteomic analysis from pericarps of guarana.$h[electronic resource]
260 $c2014
520 $aGuarana has great agricultural potential and is largely used therapeutically and in the production of non-alcoholic energy drinks. Genomic and proteomic studies are crucial to identify proteins that play central roles in the maintenance and viability of fruits, as well as to identify proteins related to the main metabolic pathways. However, the success of any protein analysis starts with the protein extract preparation, which needs to offer an extract that is free of contaminants. This study aimed to evaluate different extraction methods to obtain high-quantity and high-quality extracts that are compatible with analysis by 2-dimensional electrophoresis and tandem mass spectrometry protein identification. Three different methods were tested: trichloroacetic acid (TCA)/acetone, sodium dodecyl sulfate (SDS)/phenol, and polyvinylpolypyrrolidone (PVPP)/SDS/phenol.
650 $apolyphenols
650 $aGuaraná
650 $aPaullinia Cupana
653 $aPlant proteomics
653 $aTwo-dimensional electrophoresis
700 1 $aANGELO, P. C. da S.
700 1 $aNOGUEIRA, P. P. O.
700 1 $aGONÇALVES, J. F. C.
700 1 $aFRANCO, A. M.
700 1 $aASTOLFI-FILHO, S.
700 1 $aLÓPEZ-LOZANO, J. L.
700 1 $aANDRADE, E. V.
773 $tGenetics and Molecular Research$gv. 13, n. 3, p. 8014-8024, 2014.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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